研究業績（個人：教員：天野剛志）

3 研究業績
原著論文    H30/9/27現在
  1. Hiroaki, H.*, Satomura, K., Goda, N., Nakakura, Y., Hiranuma, M., Tenno, T., Hamada, D., Ikegami, T., 2018., Spatial overlap of claudin- and phosphatidylinositol phosphate-binding sites on the first PDZ domain of zonula occludens 1 studied by NMR, Molecules, 23(10), 2465; doi: 10.3390/molecules23102465
  2. Hori, K., Ajioka, K., Goda, N., Shindo, A., Takagishi, M., Tenno, T., and Hiroaki, H.*, 2018, Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index., Frontiers in Pharmacology. 9, Article 983, doi:3389/fphar.2018.00983
  3. Okazaki, H., Matsuo, N., Tenno, T., Goda, N., Shigemtsu, Y., Ota, M., and Hiroaki, H*, 2018, Using 1HN amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method. Protein Science, published online, doi: 1002/pro.3485.
  4. Matsuo, N., Goda, N., Shimizu, K., Tenno, T., Fukuchi, F., Ota, M. and Hiroaki, H.*, 2018, Discovery of Cryoprotective Activity of Human-genome derived Intrinsically Disordered Proteins. Int J Mol Sci,, 19(2), 401; doi: 10.3390/ijms19020401　
  5. Shigemitsu, Y., Iwaya, N., Goda, N., Matsuzaki, M., Tenno, T., Narita, A., Hoshi, M., Hiroaki, H. (2016) Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1-3,3,3-hexafluoro-2-propanol. Anal. Biochem. 498, 59-67
  6. Goda, N., Shimizu, K., Kuwahara, Y., Tenno, T., Noguchi, T., Ikegami, T., Ota, M., Hiroaki, H. (2015) A Method for Systematic Assessment of Intrinsically Disordered  Protein Region by NMR. Int. J. Mol. Sci. 16, 15743-15760
  7. Goda, N., Matsuo, N., Tenno, T., Ishino, S., Fukuchi, S., Ota, M., Hiroaki, H. (2015) An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study. Intrinsically Diord. Proteins 3(1), 1-6, DOI: 10.1080/21690707.2015.1011004
  8. Fujiwara, Y., Goda, N., Tamashiro, T., Narita, H., Satomura, K., Tenno, T., Nakagawa, A., Oda, M., Suzuki, M., Sakisaka, T., Takai, Y., Hiroaki, H. (2015) Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site. Protein Sci. 24(3), 376-385
  9. Ishino, S., Yamagami, T., Kitamura, M., Kodera, N., Mori, T., Sugiyama, S., Ando, T., Goda, N., Tenno, T., Hiroaki, H., Ishino, Y. (2014) Multiple Interactions of the Intrinsically  Disordered Region between the Helicase and Nuclease Domains of the Archaeal Hef Protein. J. Biol. Chem. 289(31), 21627-21639.
  10. Tenno, T., Goda, N., Umetsu, Y., Ota, M., Kinoshita, K., and Hiroaki, H. (2013) Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Vritual Screening. Molecules, 18, 9567-9581.
  11. Ota, M., Koike, R., Amemiya, T., Tenno, T., Romero, P.R., Hiroaki, H., Dunker, A.K., and Fukuchi, S. (2013) An assignment of intrinsically disordered regions of proteins based on NMR structures. J Structural Biol., 181:29-36.
  12. Iwaya, N., Akiyama, K., Goda, N., Tenno, T., Fujiwara, Y., Hamada, D., Ikura, T., Shirakawa, M., and Hiroaki, H. (2012) Effect of Ca2+ on the microtubule-severing enzyme p60-katanin Insight into the substrate-dependent activation mechanism. FEBS J., 279(7), 1339-1352
  13. Isogai, S., Morimoto, D., Arita, K., Unzai, S., Tenno, T., Hasegawa, J., Sou, Y.-S., Komatsu, M., Tanaka, K., Shirakawa, M., and Tochio, H. (2011) Crystal Structure of the Ubiquitin-associated (UBA) Domain of p62 and Its Interaction with Ubiquitin. J. Biol. Chem., 286(36), 31864-31874
  14. Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., and Hiroaki, H. (2011) Structure and Function of the N-terminal Nucleolin Binding Domain of Nuclear Valocin-containing Protein-like 2 (NVL2) Harboring a Nuclear Localization Signal. J. Biol. Chem., 286(24), 21732-21741
  15. Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T., and Itoh T. (2011) SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain. J. Cell Biol., 193(5), 901-916
  16. Umetsu, Y., Tenno, T., Goda, N., Shirawaka, M., Ikegami, T., and Hiroaki, H. (2011) Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments. Biochim. Biophys. Acta, 1814(5), 724-730
  17. Morimoto, D., Isogai, S., Tenno, T., Tochio, H., Shirakawa, M., Ariyoshi, M. (2010) Purification, crystallization and preliminary crystallographic studies of Lys48-linked polyubiquitin chains. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66, 834-837
  18. Igarashi, R., Sakai, T., Hara, H., Tenno, T., Tanaka, T., Tochio, H., Shirakawa, M. (2010) Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments. J. Am. Chem. Soc. 132, 8228-8229
  19. Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H. (2010) A Common Substrate Recognition Mode Conserved between Katanin p60 and VPS4 Governs Microtuble Severing and Membrane Skeleton Reorganization. J. Biol. Chem. 285, 16822-16829
  20. Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M. (2009) High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. Nature, 458, 106-109
  21. Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H. (2008) Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding protein 3 and 5. Exp. Cell Res. 314, 2352-2361
  22. Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., and Shirakawa, M. (2007) Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP. Anal. Biochem.371, 247-249
  23. Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H. (2007) LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells. Biochim. Biophys. Acta 1773, 141-146
  24. Hara, H., Tenno, T., and Shirakawa, M. (2007) Distance determination in human ubiquitin by pulsed double electron-electron resonance and double quantum coherence ESR methods. J. Magn. Reson. 184, 78-84
  25. Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., and Shirakawa, M. (2006) In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J. Biomol. NMR, 36, 179-188
  26. Ohno, A., Jee, J.-G., Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., and Shirakawa, M. (2005) Structure of the UBA Domain of Dsk2p in Complex with Ubiquitin: Molecular Determinants for Ubiquitin Recognition.Structure, 13, 521-532
  27. Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E. H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., and Shirakawa, M. (2004) Structural basis for distinct roles of Lys 63- and Lys48-linked polyubiquitin chains. Genes Cells, 9, 865-875
  28. Nomura, M., Kobayashi, T., Kohono, T., Fujiwara, K., Tenno, T., Shirakawa, M., Ishizaki, I., Yamamoto, K., Matsuyama, T., Mishima, M., Kojima, C. (2004) Paramagnetic NMR study of Cu2+-IDA complex localization on a protein surface and its application to elucidate long distance information. FEBS Lett., 566(1-3), 157-161
  29. Tenno, T., Goda, N., Tateishi, Y., Tochio, T., Mishima, M., Hayashi, H., Shirakawa, M., and Hiroaki, H. (2004) High-throughput construction method of expression vector of peptides for NMR study suited for isotopic labeling. Protein Eng. Des. Sel., 17, 305-314
  30. Goda, N., Tenno, T., Takasu, H., Hiroaki, H., and Shirakawa, M. (2004) The PRESAT-vector: Asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics. Protein Sci., 13, 652-658
  31. Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J.-G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., and Shirakawa M. (2004) Structure of the Ubiquitin-interacting Motif of S5a Bound to the Ubiquitin-like Domain of HR23B. J. Biol. Chem.,279, 4760-4767
日本語総説
  • 天野剛志、廣明秀一（2013）「立体構造が明らかにしたGタンパク質共役受容体の刺激受容のしくみ」領域融合レビュー　2, e003
  • 天野剛志、廣明秀一、白川昌宏（2008）「クラスB GPCR細胞外ドメインのペプチドリガンド分子認識機構」　生化学　80, 948-958
  • 天野剛志、廣明秀一　（2008）「進むG蛋白質共役型受容体の立体構造解析：展望と課題」　蛋白質核酸酵素　53, 256-264
  • 天野剛志、合田名都子、廣明秀一（2006）Close Up実験法「高効率かつ高速な融合タンパク質発現ベクターの構築法」　実験医学　24, 1675-1680
  • 天野剛志、合田名都子、廣明秀一　(2004)「蛋白質ドメイン研究のための迅速なベクター構築法」　蛋白質核酸酵素　19, 2587-2594
著書
  • 竹縄忠臣・伊藤俊樹編（2011）実験医学別冊　実験ハンドブックシリーズ改訂　タンパク質実験ハンドブック　羊土社
  • 担当項目　第3章　III）ゲル濾過クロマトグラフィー、V）リガンドアフィニティークロマトグラフィー、VI）金属アフィニティークロマトグラフィー、VII）抗体アフィニティークロマトグラフィー（p60-64、p68-83）
特許
  • 特許第4487036号　「新規ベクター及びその利用」
  • 特許番号No.1669445（フランス)
  • 特許番号60 2004 017 488.2-08（ドイツ）
発明者　廣明秀一、合田名都子、天野剛志
出願日　2004/8/31
Updated: 2018/09/27 — 17:59