原著論文(分野全体)

2018

  • Hiroaki, H.*, Satomura, K., Goda, N., Nakakura, Y., Hiranuma, M., Tenno, T., Hamada, D., Ikegami, T., 2018., Spatial overlap of claudin- and phosphatidylinositol phosphate-binding sites on the first PDZ domain of zonula occludens 1 studied by NMR, Molecules, 23(10), 2465; doi: 10.3390/molecules23102465
  • Hori, K., Ajioka, K., Goda, N., Shindo, A., Takagishi, M., Tenno, T., and Hiroaki, H.*, 2018, Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index., Frontiers in Pharmacology. 9, Article 983, doi:3389/fphar.2018.00983
  • Okazaki, H., Matsuo, N., Tenno, T., Goda, N., Shigemtsu, Y., Ota, M., and Hiroaki, H*, 2018, Using 1HN amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method. Protein Science, published online, doi: 1002/pro.3485.
  • Matsuo, N., Goda, N., Shimizu, K., Tenno, T., Fukuchi, F., Ota, M. and Hiroaki, H.*, 2018, Discovery of Cryoprotective Activity of Human-genome derived Intrinsically Disordered Proteins. Int J Mol Sci,, 19(2), 401; doi: 10.3390/ijms19020401

2017

  • Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H. and Ueno, Y.*, 2017, Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity. RSC Advances., 7, 25378-25386. DOI: 10.1039/C7RA03896H
  • Shimasaki, T., Ohtsuka, H., Naito, C., Tenno, T., Hiroaki, H., Murakami, H., Hirofumi Aiba, H*. 2017, Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast. Molecular Genetics and Genomics, 292(2):475-481. DOI: 10.1007/s00438-016-1285-x

2016

  • Shigemitsu, Y., Iwaya, N., Goda, N., Matsuzaki, M., Abe., Y., Narita, A., Tenno, T., Hoshi, M., and Hiroaki, H.* 2016, Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1–42 in 1,1,1,3,3,3-hexafluoro-2-propanol. Analytical Biochemistry, (2016) Accepted. DOI: 10.1016/j.ab.2015.12.021

2015

  • Ohnishi, T., Yanazawa, M., Kitamura, Y., Sasahara, T., Nishiyama, T., Komura, H., Hiroaki, H., Fukazawa, Y., Kii, I., Kakita, A., Takeuchi, A., Ito, A., Takeda, H., Hirao, H., Inoue, M., Muramatsu, S., Matsui, K., Tada, M., Sato, M., Goda, N., Takino, N., Sakai, S., Arai, Y., Umetsu, Y., Takahashi, H., Hagiwara, M., Sawasaki, T., Iwasaki, G., Nakamura, Y., Nabeshima, Y., Teplow, T. B., and Hoshi, M.* 2015, Na,K-ATPasea3 Is a Death Target of Alzheimer Patient Amyloid-b Assembly. Proc. Natl. Acad. Sci. U.S.A., (2015), 112(32):E4465–E4474. DOI: 10.1073/pnas.1421182112
  • Goda, N., Shimizu, K., Kuwahara, Y., Tenno, T., Noguchi,T., Ikegami, T., Ota, M., and Hiroaki, H*., A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR. (2015), Int J Mol Sci, 16 (7):15743-15760. DOI: 10.3390/ijms160715743
  • Goda, N., Matsuo, N., Tenno, T., Ishino, S., Ishino, Y., Fukuchi, S., Ota, M., and Hiroaki, H*. 2015, An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study. Intrinsically Disordere Proteins, (2015) 3(1):1-6. DOI: 1080/21690707.2015.1011004
  • Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H.*, Crystal structure of afadin PDZ domain–nectin-3 complex shows the structural plasticity of the extended ligand-binding site. Protein Sci., (2015). 24(3):376-385 DOI: 10.1002/pro.2628

2014

  • Kobayashi, Y., Tsutsumi, H., Abe, T., Ikeda, K., Tashiro, Y., Unzai, S., Kamikubo,H., Kataoka, M., Hiroaki, H., and Hamada, D*.,  Decreased amyloidogenicity by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain. Biochemistry, (2014), accepted. DOI: 10.1021/bi5007892
  • Ishino, S., Yamagami, T., Kitamura, M., Kodera, N., Mori, T., Sugiyama, S., Ando, T., Goda, N., Tenno, T., Hiroaki, H., and Ishino, Y*., Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein. J Biol Chem (2014), accepted. doi: 10.1074/jbc.M114.554998
  • Fukuchi, S*., Amemiya, T., Sakamoto, S., Nobe, Y., Hosoda, K., Kado, Y., Murakami, D. S., Koike, R., Hiroaki, H., and Ota M*. 2013., IDEAL in 2014 illustrates interaction network composed of intrinsically disordered proteins and their binding partners. Nucleic Acids Res., 42:D320-325. doi: 10.1093/nar/gkt1010.

2013

  • Tenno, T., Goda, N., Umetsu, Y., Ota, M., Kinoshita, K. and Hiroaki, H.* 2013. Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening. Molecules 18, 9567-9581. [open access]
  • Iwaya, N., Takasu, H., Goda, N., Shirakawa, M., Tanaka, T., Hamada, D., and Hiroaki, H*. 2013. MIT Domain is a Ca2+-dependent Phosphoinositide-binding domain. J. Biochem., 153(5):473-481.
  • Ota, M*., Koike, R., Amemiya, T., Tenno, T., Romero, P.R., Hiroaki, H., Dunker, A.K., and Fukuchi, S. 2013. An assignment of intrinsically disordered regions of proteins based on NMR structures. J Structural Biol., 181:29-36.

2012

  • Yahara A, Shrestha AR, Yamamoto T, Hari Y, Osawa T, Yamaguchi M, Nishida M, Kodama T, Obika S*., 2012,  Amido-bridged nucleic acids (AmNAs): synthesis, duplex stability, nuclease resistance, and in vitro antisense potency. Chembiochem. 13(17):2513-2516.
  • Wheeler M, Chardon A, Goubet A, Morihiro K, Tsan SY, Edwards SL, Kodama T, Obika S, Veedu RN*. 2012, Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases. Chem Commun (Camb)48(89):11020-11022.
  • Rahman SM, Baba T, Kodama T, Islam MA, Obika S*. 2012. Hybridizing ability and nuclease resistance profile of backbone modified cationic phosphorothioate oligonucleotides. 2012. Bioorg Med Chem. 20(13):4098-4102.
  • Kawada Y, Kodama T, Miyashita K, Imanishi T, Obika S. Synthesis and evaluation of novel caged DNA alkylating agents bearing 3,4-epoxypiperidine structure. 2012. Org Biomol Chem.  10(26):5102-5108
  • Iwaya, N., Akiyama, K., Goda, N., Tenno, T., Fujiwara, Y., Hamada, D., Ikura, T., Shirakawa, M., and Hiroaki, H*. 2012. Effect of Ca2+ on microtubule severing enzyme katanin p60; insight into the substrate dependent activation mechanism. FEBS J., 279, (7), 1339-1352.
  • Fukuchi, S*., Sakamoto, S., Nobe, Y., Murakami, D. S., Amemiya, T., Hosoda, K., Koike, R. Hiroaki, H., and Ota, M*., 2012. IDEAL – Intrinsically Disordered proteins with Extensive Annotations and Literature. Nucleic Acids Research (database issue),. 40 (1):D507-511. (Epub 2011 Nov 8.)

2011

  • Hiroaki, H*., Umetsu, Y., Hoshi, M., Nabeshima, Y. and Kohda, D., 2011. A Simplified Recipe for Assigning Amide NMR Signals Using Combinatorial 14N Amino Acid Inverse-Labeling. J Structural Functional Genomics., 12 (3): 167-174.
  • Isogai S, Morimoto D, Arita K, Unzai S, Tenno T, Hasegawa J, Sou YS, Komatsu M, Tanaka K, Shirakawa M*, Tochio H*., 2011. Crystal structure of the ubiquitin-associated (UBA) domain of p62 and its interaction with ubiquitin. J Biol Chem., 286, (36), 31864-31874.
  • Tsuruta, T., Umetsu, Y., Iwaya, N., Taniguchi, R., Goda, N.,  Tenno, T., Kuwahara, Y., and Hiroaki, H*., 2011. 1H, 13C, and 15N resonance assignment of the SPFH domain of human stomatin. Biomol. NMR Assign,5, in press.
  • Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T. Itoh, T*. 2011. SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide–binding domain. J Cell Biol 193 (5):901-916.
  • Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., Hiroaki, H*. 2011. Structure and function of the N-terminal nucleolin binding domain of nuclear Valocin containing protein like 2 (NVL2) harboring a nucleolar localization signal. 2011. J Biol Chem 286 (24):21732-21741.
  • Umetsu, Y., Taniguchi, R., Satomura, R., Goda, N., Ikegami, T., Furuse, M., and Hiroaki, H*. 2011. 1H, 13C, and 15N resonance assignment of the first PDZ domain of mouse ZO-1. Biomol. NMR Assign 5 (2): 207-210.
  • Umetsu, Y, Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., and Hiroaki, H*. 2011. Structural difference of vasoactive intestinal peptide (VIP) in two distinct membrane mimicking conditions., BBA-Proteins Proteomics. 1814:724-730.

2010

  • Motono, C., Nakata, J., Koike, R., Shimizu, K., Shirota, M., Amemiya, T., Tomii, K., Nagano, N., Sakaya, N., Misoo, K., Sato, M., Kidera, A., Hiroaki, H., Shirai, T., Kinoshita, K., Noguchi, T., and Ota, M. 2010. SAHG, a comprehensive database of predicted structures of all human proteins. Nucleic Acids Res 39(suppl 1): D487-D493.
  • Nishimasu, R., Komori, H., Higuchi, Y., Nishimasu, H., and Hiroaki, H*. 2010. Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3. Kobe J Med Sci 56: E125-E139.
  • Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M. 2010. 1H, 13C, and 15N resonance assignment of the TIR domain of human MyD88. Biomol NMR Assign 4: 123-125.
  • Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H*. 2010. A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization. J Biol Chem 285: 16822-16829.
  • Jee, J., Mizuno, T., Kamada, K., Tochio, H., Chiba, Y., Yanagi, K., Yasuda, G., Hiroaki, H., Hanaoka, F., and Shirakawa, M. 2010. Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins. J Biol Chem 285: 15931-15940.

2009

  • Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H*. 2009. Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii. Biophys J 97: 2034-2043.
  • Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M. 2009. High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. Nature 458: 106-109.
  • Ohnishi, H., Tochio, H., Kato, Z., Orii, K. E., Li, A., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M. 2009. Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling. Proc Natl Acad Sci U S A 106: 10260-10265.

2008

  • Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H*.  008. The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold. Protein Sci 17: 1915-1924.
  • Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., and Shirakawa, M. 2008. Kinetic and thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA. Biochemistry-US 47: 3266-3271.
  • Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H*. 2008. Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5. Exp Cell Res 314:  2352-2361.
  • Tomida, J., Masuda, Y., Hiroaki, H., Ishikawa, T., Song, I., Tsurimoto, T., Tateishi, S., Shiomi, T., Kamei, Y., Kim, J., Kamiya, K., Vaziri, C., Ohmori, H., and Todo, T. 2008. DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex. J Biol Chem 283: 9071-9079.

2007

  •  Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., and Hiroaki,  H*. 2007. Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. J Biomol NMR 37: 53-63.
  • Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H*. 2007.LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells. Biochim Biophys Acta-Mol Cell Res 1773: 141-146.
  • Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H*., and Shirakawa, M. 2007. Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP. Anal Biochem 371: 247-249.
Updated: 2018/09/27 — 18:02