原著論文（分野全体）

2021

• Naoki Matsuo#, Natsuko Goda#, Takeshi Tenno, and Hidekazu Hiroaki*. 2021, Cryoprotective activities of FK20, a human genome-derived intrinsically disordered peptide against cryosensitive enzymes without a stereospecific molecular interaction., Peer J Physical Chemistry, 3:e20, doi; 10.7717/peerj-pchem.20, https://peerj.com/articles/pchem-20/#
• Takeshi Tenno, Kohki kataoka, Natsuko Goda, Hidekazu Hiroaki*, 2021, NMR-guided repositioning of non-steroidal anti-inflammatory drugs into tight junction modulators. International Journal of molecular Sciences, 22(5), 2583
• Masaki Nakano, Yachiyo Mitsuishi, Lei Liu, Naoki Watanabe, Emi Hibino, Saori Hata, Takashi Saito, Takaomi C Saido, Shigeo Murayama, Kensaku Kasuga, Takeshi Ikeuchi, Toshiharu Suzuki, Masaki Nishimura, 2021, Extracellular Release of ILEI/FAM3C and Amyloid-β Is Associated with the Activation of Distinct Synapse Subpopulations, Journal of Alzheimer’s Disease, 80(1), 159-174 DOI: 10.3233/JAD-201174

2020

• Misaki Hisada, Minami Hiranuma, Mio Nakashima, Natsuko Goda, Takeshi Tenno, Hidekazu Hiroaki, 2020, High dose of baicalin or baicalein can reduce tight junction integrity by partly targeting the first PDZ domain of zonula occludens-1 (ZO-1). European journal of pharmacology, 887, 173436. doi: 10.1016/j.ejphar.2020.173436.
• Mio Nakashima, Misaki Hisada, Natsuko Goda, Takeshi Tenno, Ayaka Kotake, Yuko Inotsume, Ikuo Kameoka, Hidekazu Hiroaki, 2020,  Opposing effect of naringenin and quercetin on the junctional compartment of MDCK II cells to modulate the tight junction. Nutrients, 12(11) 3285. doi: 10.3390/nu12113285
• Naoko Iwaya, Natsuko Goda, Mizuki Matsuzaki, Akihiro Narita, Yoshiki Shigemitsu, Takeshi Tenno, Yoshito Abe, Minako Hoshi, Hidekazu Hiroaki, 2020, Principal Component Analysis of Data from NMR Titration Experiment of Uniformly 15N Labelled Amyloid Beta (1–42) Peptide with Osmolytes and Phenolic Compounds. Archives of Biochemistry and Biophysics, 690, 108446. doi: 10.1016/j.abb.2020.108446
• Masaki Nishimura, Naoki Watanabe, Emi Hibino, Masaki Nakano, Yachiyo Mitsuishi, Lei Liu, Takuma Sugi, 2020, FAM3C in Alzheimer’s disease. A risk-related molecule and potential therapeutic target. Neuroscience of Dementia, 2, 293-307 doi: 10.1016/B978-0-12-815868-5.00019-0
• Koki Ikeda, Suzuki Shota, Yoshiki Shigemitsu, Takeshi Tenno, Natsuko Goda, Atsunori Oshima, Hidekazu Hiroaki, 2020, Presence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of Aβ(1–42) in amino acid sequence independent manner. Scientific Reports, 10(1), 12334. doi: 10.1038/s41598-020-69129-1
• Emi Hibino, Masaru Hoshino, 2020, A novel mode of interaction between intrinsically disordered proteins. Biophysics and Physicobiology /Biophysical Society of Japan 17 86-93. doi: 10.2142/biophysico.BSJ-2020012. (review)

2019

• Naoko Takenaga, Takumi Hayashi, Shohei Ueda, Hiroyuki Satake, Yoichi Yamada, Tetsuya Kodama, Toshifumi. Dohi., 2019, Synthesis of Uracil-Iodonium(III) Salts for Practical Utilization as Nucleobase Synthetic Modules. Molecules, 24(17), 3034, doi:10.3390/molecules24173034
• Yoshihiro Moai , Hidekazu Hiroaki, Saoshi Obika, Tetsuya Kodama*., 2019, Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base, Nucleosides, Nucleotides & Nucleic Acids, 39,131-140;  DOI: 10.1080/15257770.2019.1675169.

2018

• Hiroaki, H.*, Satomura, K., Goda, N., Nakakura, Y., Hiranuma, M., Tenno, T., Hamada, D., Ikegami, T., 2018, Spatial overlap of claudin- and phosphatidylinositol phosphate-binding sites on the first PDZ domain of zonula occludens 1 studied by NMR, Molecules, 23(10), 2465; doi: 10.3390/molecules23102465
• Hori, K., Ajioka, K., Goda, N., Shindo, A., Takagishi, M., Tenno, T., and Hiroaki, H.*, 2018, Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index., Frontiers in Pharmacology. 9, Article 983, doi:3389/fphar.2018.00983
• Okazaki, H., Matsuo, N., Tenno, T., Goda, N., Shigemtsu, Y., Ota, M., and Hiroaki, H*, 2018, Using ¹H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method. Protein Science, published online, doi: 1002/pro.3485.
• Matsuo, N., Goda, N., Shimizu, K., Tenno, T., Fukuchi, F., Ota, M. and Hiroaki, H.*, 2018, Discovery of Cryoprotective Activity of Human-genome derived Intrinsically Disordered Proteins. Int J Mol Sci,, 19(2), 401; doi: 10.3390/ijms19020401

2017

• Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H. and Ueno, Y.*, 2017, Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity. RSC Advances., 7, 25378-25386. DOI: 10.1039/C7RA03896H
• Shimasaki, T., Ohtsuka, H., Naito, C., Tenno, T., Hiroaki, H., Murakami, H., Hirofumi Aiba, H*. 2017, Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast. Molecular Genetics and Genomics, 292(2):475-481. DOI: 10.1007/s00438-016-1285-x

2016

• Shigemitsu, Y., Iwaya, N., Goda, N., Matsuzaki, M., Abe., Y., Narita, A., Tenno, T., Hoshi, M., and Hiroaki, H.* 2016, Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1–42 in 1,1,1,3,3,3-hexafluoro-2-propanol. Analytical Biochemistry, (2016) Accepted. DOI: 10.1016/j.ab.2015.12.021

2015

• Ohnishi, T., Yanazawa, M., Kitamura, Y., Sasahara, T., Nishiyama, T., Komura, H., Hiroaki, H., Fukazawa, Y., Kii, I., Kakita, A., Takeuchi, A., Ito, A., Takeda, H., Hirao, H., Inoue, M., Muramatsu, S., Matsui, K., Tada, M., Sato, M., Goda, N., Takino, N., Sakai, S., Arai, Y., Umetsu, Y., Takahashi, H., Hagiwara, M., Sawasaki, T., Iwasaki, G., Nakamura, Y., Nabeshima, Y., Teplow, T. B., and Hoshi, M.* 2015, Na,K-ATPasea3 Is a Death Target of Alzheimer Patient Amyloid-b Assembly. Proc. Natl. Acad. Sci. U.S.A., (2015), 112(32):E4465–E4474. DOI: 10.1073/pnas.1421182112
• Goda, N., Shimizu, K., Kuwahara, Y., Tenno, T., Noguchi,T., Ikegami, T., Ota, M., and Hiroaki, H*., A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR. (2015), Int J Mol Sci,　16 (7):15743-15760. DOI: 10.3390/ijms160715743
• Goda, N., Matsuo, N., Tenno, T., Ishino, S., Ishino, Y., Fukuchi, S., Ota, M., and Hiroaki, H*. 2015, An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study. Intrinsically Disordere Proteins, (2015) 3(1):1-6. DOI:　1080/21690707.2015.1011004
• Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H.*, Crystal structure of afadin PDZ domain–nectin-3 complex shows the structural plasticity of the extended ligand-binding site. Protein Sci., (2015). 24(3):376-385 DOI: 10.1002/pro.2628

2014

• Kobayashi, Y., Tsutsumi, H., Abe, T., Ikeda, K., Tashiro, Y., Unzai, S., Kamikubo,H., Kataoka, M., Hiroaki, H., and Hamada, D*.,　 Decreased amyloidogenicity by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain. Biochemistry, (2014), accepted. DOI: 10.1021/bi5007892
• Ishino, S., Yamagami, T., Kitamura, M., Kodera, N., Mori, T., Sugiyama, S., Ando, T., Goda, N., Tenno, T., Hiroaki, H., and Ishino, Y*., Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein. J Biol Chem (2014), accepted. doi: 10.1074/jbc.M114.554998
• Fukuchi, S*., Amemiya, T., Sakamoto, S., Nobe, Y., Hosoda, K., Kado, Y., Murakami, D. S., Koike, R., Hiroaki, H., and Ota M*. 2013., IDEAL in 2014 illustrates interaction network composed of intrinsically disordered proteins and their binding partners. Nucleic Acids Res., 42:D320-325. doi: 10.1093/nar/gkt1010.

2013

• Tenno, T., Goda, N., Umetsu, Y., Ota, M., Kinoshita, K. and Hiroaki, H.* 2013. Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening. Molecules 18, 9567-9581. [open access]
• Iwaya, N., Takasu, H., Goda, N., Shirakawa, M., Tanaka, T., Hamada, D., and Hiroaki, H*. 2013. MIT Domain is a Ca²⁺-dependent Phosphoinositide-binding domain. J. Biochem., 153(5):473-481.
• Ota, M*., Koike, R., Amemiya, T., Tenno, T., Romero, P.R., Hiroaki, H., Dunker, A.K., and Fukuchi, S. 2013. An assignment of intrinsically disordered regions of proteins based on NMR structures. J Structural Biol., 181:29-36.

2012

2011

• Hiroaki, H*., Umetsu, Y., Hoshi, M., Nabeshima, Y. and Kohda, D., 2011. A Simplified Recipe for Assigning Amide NMR Signals Using Combinatorial ¹⁴N Amino Acid Inverse-Labeling. J Structural Functional Genomics., 12 (3): 167-174.
• Isogai S, Morimoto D, Arita K, Unzai S, Tenno T, Hasegawa J, Sou YS, Komatsu M, Tanaka K, Shirakawa M*, Tochio H*., 2011. Crystal structure of the ubiquitin-associated (UBA) domain of p62 and its interaction with ubiquitin. J Biol Chem., 286, (36), 31864-31874.
• Tsuruta, T., Umetsu, Y., Iwaya, N., Taniguchi, R., Goda, N.,  Tenno, T., Kuwahara, Y., and Hiroaki, H*., 2011. ¹H, ¹³C, and ¹⁵N resonance assignment of the SPFH domain of human stomatin. Biomol. NMR Assign,5, in press.
• Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T. Itoh, T*. 2011. SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide–binding domain. J Cell Biol 193 (5):901-916.
• Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., Hiroaki, H*. 2011. Structure and function of the N-terminal nucleolin binding domain of nuclear Valocin containing protein like 2 (NVL2) harboring a nucleolar localization signal. 2011. J Biol Chem 286 (24):21732-21741.
• Umetsu, Y., Taniguchi, R., Satomura, R., Goda, N., Ikegami, T., Furuse, M., and Hiroaki, H*. 2011. ¹H, ¹³C, and ¹⁵N resonance assignment of the first PDZ domain of mouse ZO-1. Biomol. NMR Assign 5 (2): 207-210.
• Umetsu, Y, Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., and Hiroaki, H*. 2011. Structural difference of vasoactive intestinal peptide (VIP) in two distinct membrane mimicking conditions., BBA-Proteins Proteomics. 1814:724-730.

2010

• Motono, C., Nakata, J., Koike, R., Shimizu, K., Shirota, M., Amemiya, T., Tomii, K., Nagano, N., Sakaya, N., Misoo, K., Sato, M., Kidera, A., Hiroaki, H., Shirai, T., Kinoshita, K., Noguchi, T., and Ota, M. 2010. SAHG, a comprehensive database of predicted structures of all human proteins. Nucleic Acids Res 39(suppl 1): D487-D493.
• Nishimasu, R., Komori, H., Higuchi, Y., Nishimasu, H., and Hiroaki, H*. 2010. Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3. Kobe J Med Sci 56: E125-E139.
• Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M. 2010. ¹H, ¹³C, and ¹⁵N resonance assignment of the TIR domain of human MyD88. Biomol NMR Assign 4: 123-125.
• Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H*. 2010. A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization. J Biol Chem 285: 16822-16829.
• Jee, J., Mizuno, T., Kamada, K., Tochio, H., Chiba, Y., Yanagi, K., Yasuda, G., Hiroaki, H., Hanaoka, F., and Shirakawa, M. 2010. Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins. J Biol Chem 285: 15931-15940.

2009

• Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H*. 2009. Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii. Biophys J 97: 2034-2043.
• Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M. 2009. High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. Nature 458: 106-109.
• Ohnishi, H., Tochio, H., Kato, Z., Orii, K. E., Li, A., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M. 2009. Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling. Proc Natl Acad Sci U S A 106: 10260-10265.

2008

• Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H*.  008. The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold. Protein Sci 17: 1915-1924.
• Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., and Shirakawa, M. 2008. Kinetic and thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA. Biochemistry-US 47: 3266-3271.
• Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H*. 2008. Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5. Exp Cell Res 314:  2352-2361.
• Tomida, J., Masuda, Y., Hiroaki, H., Ishikawa, T., Song, I., Tsurimoto, T., Tateishi, S., Shiomi, T., Kamei, Y., Kim, J., Kamiya, K., Vaziri, C., Ohmori, H., and Todo, T. 2008. DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex. J Biol Chem 283: 9071-9079.

2007

•  Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., and Hiroaki,  H*. 2007. Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. J Biomol NMR 37: 53-63.
• Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H*. 2007.LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells. Biochim Biophys Acta-Mol Cell Res 1773: 141-146.
• Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H*., and Shirakawa, M. 2007. Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP. Anal Biochem 371: 247-249.