原著論文 (2006年以前)

2006

•  Shiozawa, K., Goda, N., Shimizu, T., Mizuguchi, K., Kondo, N., Shimozawa, N., Shirakawa, M., and Hiroaki,  H*. 2006. The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97. FEBS J 273: 4959-4971.
• Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., and Shirakawa, M. 2006. In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J Biomol NMR 36: 179-188.
• Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki,
  H., and Shirakawa, M. 2006. Crystal structure of SUMO-3-modified thymine-DNA glycosylase. J Mol Biol 359: 137-147.

2005

•  Sakurai, Y., Mizuno, T., Hiroaki, H., Gohda, K., Oku, J., and Tanaka, T. 2005. High thermal stability imparted by a designed tandem Arg-Trp stretch in an alpha-helical coiled coil. Angew Chem Int Ed Engl 44: 6180-6183.
• Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M. 2005. Crystal structure of thymine DNA glycosylase conjugated to SUMO-1. Nature 435: 979-982.
• Sakurai, Y., Mizuno, T., Hiroaki, H., Oku, J. I., and Tanaka, T. 2005. Optimization of aromatic side chain size complementarity in the hydrophobic core  of a designed coiled-coil. J Pept Res 66: 387-394. 
• Takasu, H., Jee, J. G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., and Hiroaki, H*. 2005. Structural characterization of the MIT domain from human Vps4b. Biochem Biophys Res Commun 334: 460-465. 
• Ohno, A., Jee, J., Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., and Shirakawa, M. 2005. Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition. Structure 13: 521-532.

~2004

•  Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H*. 2004. Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain. J Biol Chem 279: 50060-50068.
• Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E. H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., and Shirakawa, M. 2004. Structural basis for distinct roles of Lys63- and Lys48-linked polyubiquitin chains. Genes Cells 9: 865-875.
• Tanaka, T., Mizuno, T., Fukui, S., Hiroaki, H., Oku, J., Kanaori, K., Tajima, K., and Shirakawa, M. 2004. Two-metal ion, Ni(II) and Cu(II), binding alpha-helical coiled coil peptide. J Am Chem Soc 126: 14023-14028.
• Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J. G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., and Shirakawa, M. 2004. Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B. J Biol Chem 279: 4760-4767.
• Goda, N., Tenno, T., Takasu, H., Hiroaki, H*., and Shirakawa, M. 2004. The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics. Protein Sci 13: 652-658.
• Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H*. 2004. Crystallographic characterization of the N-terminal domain of PEX1. Acta Crystallogr D Biol Crystallogr 60: 2098-2099.
• Tenno, T., Goda, N., Tateishi, Y., Tochio, H., Mishima, M., Hayashi, H., Shirakawa, M., and Hiroaki, H*. 2004. High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling. Protein Eng Des Sel 17: 305-314.
• Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M. 2003. Thermodynamic study of hybridization properties of heterochiral nucleic acids. Biochem Biophys Res Commun 309: 79-83.
• Shimizu, M., Hiroaki, H*., Kohda, D., Morita, E.H., Hotta, S., and Morikawa, K. 2003. ¹H, ¹³C and ¹⁵N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein. J Biomol NMR 26: 277-278. 
• Ago, T., Kuribayashi, F., Hiroaki, H., Takeya, R., Ito, T., Kohda, D., and Sumimoto, H. 2003. Phosphorylation of p47^phox directs phox homology domain from SH3 domain toward phosphoinositides, leading to phagocyte NADPH oxidase activation. Proc Natl Acad Sci U S A 100: 4474-4479.
• Shimizu, M., Hiroaki, H., Kohda, D., Hosoya, T., Akiyama-Oda, Y., Hotta, Y., Morita, E. H., and Morikawa, K. 2003. NMR and ICP spectroscopic analysis of the DNA-binding domain of the Drosophila GCM protein reveals a novel Zn²⁺ -binding motif. Protein Eng 16: 247-254. 
• Ago, T., Takeya, R., Hiroaki, H., Kuribayashi, F., Ito, T., Kohda, D., and Sumimoto, H. 2001. The PX domain as a novel phosphoinositide- binding module. Biochem Biophys Res Commun 287: 733-738.
• Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., and Kohda, D. 2001. Solution structure of the PX domain, a target of the SH3 domain. Nat Struct Biol 8: 526-530. 
• Li, X., Suzuki, K., Kanaori, K., Tajima, K., Kashiwada, A., Hiroaki, H., Kohda, D., and Tanaka, T. 2000. Soft metal ions, Cd(II) and Hg(II), induce triple-stranded alpha-helical assembly and folding of a de novo designed peptide in their trigonal geometries. Protein Sci 9: 1327-1333.
• Kashiwada, A., Hiroaki, H., Kohda, D., Nango, M. , and Tanaka, T. 2000. Design of a heterotrimeric alpha-helical bundle by hydrophobic core engineering. J Am Chem Soc 122: 212-215.
• Hiroaki, Y., Mitsuoka, K., Cheng, Y., Hiroaki, H., and Fujiyoshi, Y. 1999. Influence of various nucleotides on the in situ crystallization of Ca²⁺-ATPase. Biochim Biophys Acta-Biomembrane 1415: 361-368. 
• Tanaka, Y., Fujii, S., Hiroaki, H., Sakata, T., Tanaka, T., Uesugi, S., Tomita, K., and Kyogoku, Y. 1999. A’-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC). Nucleic Acids Res 27: 949-955. 
• Suzuki, K., Hiroaki, H., Kohda, D., and Tanaka, T. 1998. An isoleucine zipper peptide forms a native-like triple stranded coiled coil in solution. Protein Eng 11: 1051-1055. 
• Suzuki, K., Hiroaki, H., Kohda, D., Nakamura, H., and Tanaka, T 1998. Metal ion induced self-assembly of a designed peptide into a triple-stranded alpha-helical bundle: A novel metal binding site in the hydrophobic core. J Am Chem Soc 120: 13008-13015. 
• Hiroaki, H., Klaus, W., and Senn, H. 1996. Determination of the solution structure of the SH3 domain of human p56 Lck tyrosine kinase. J Biomol NMR 8: 105-122. 
• Iwamoto, T., Hiroaki, H., Furuichi, Y., Wada, K., Satoh, M., Satoh, M., Osada, T., and Gagnon, C. 1995. Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and  codes for a sperm motility inhibitor protein. FEBS Lett 368: 420-424. 
• Urata, H., Urata, M., Akagi, M., Hiroaki, H., And Uesugi, S. 1994. Spectroscopic Characterization Of The Decadeoxynucleotide Duplex Modified With Dichloroethylenediamineplatinum(Ii). Nucleosides & Nucleotides 13: 1259-1269. 
• Odai, O., Hiroaki, H., Tanaka, T., And Uesugi, S. 1994. Properties Of A Hammerhead-Type Rna Enzyme-System That Consists Of 3 Rna Oligomer Strands. Nucleosides & Nucleotides 13: 1569-1579. 
• Itoh, T., Tomita, K., Hakoshima, T., Hiroaki, H., Uesugi, S., Nishikawa, S., Amisaki, T., Morioka, H., Ohtsuka, E., and Ikehara, M. 1991. Conformation of 2’GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods. J Biochem (Tokyo) 110: 677-680. 
• Hiroaki, H., Nakayama, T., Ikehara, M., and Uesugi, S. 1991. Interaction of bleomycin with deoxyribonucleic acid oligomer: proton nuclear magnetic resonance titration study using novel bleomycin complexes with Ni²⁺ and  VO³⁺. Chem Pharm Bull (Tokyo) 39: 2780-2786. 
• Odai, O., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S. 1990. The role of a conserved guanosine residue in the hammerhead-type RNA enzyme. FEBS Lett 267: 150-152. 
• Sakata, T., Hiroaki, H., Oda, Y., Tanaka, T., Ikehara, M., and Uesugi, S. 1990. Studies on the structure and stabilizing factor of the CUUCGG hairpin RNA using chemically synthesized oligonucleotides. Nucleic Acids Res 18: 3831-3839. 
• Odai, O., Kodama, H., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S. 1990. Synthesis and NMR study of ribo-oligonucleotides forming a hammerhead-type RNA enzyme system. Nucleic Acids Res 18: 5955-5960. 
• Urata, H., Yamamoto, K., Akagi, M., Hiroaki, H., and Uesugi, S. 1989. A 2-deoxyribonolactone-containing nucleotide: isolation and characterization of the alkali-sensitive photoproduct of the trideoxyribonucleotide d(ApCpA). Biochemistry-US 28: 9566-9569. 
• Hiroaki, H., and Uesugi, S*. 1989. Novel ¹H-³¹P heteronuclear coherence transfer spectroscopy based on spin locking  MLEV-17 and its application to signal assignment for a short DNA fragment. FEBS Lett 244: 43-46.